Using Pap Pens for IHC

What to do and how to use Pap Pens for IHC?

Do’s

  • Work only on a dry slides. The Gnomepen will overcome slightly damp slides but will not be able to adhere to a wet or water covered glass slide.
  • Use a fixative such as Paraformaldehyde or Formalin.
  • The GnomePen formulation is highly resistant to Tween20, Triton X100 and X114. Even overnight incubations are optional.
  • If you insist on removing your Hydrophobic barrier before applying a cover slip (not necessary!) use a q-tip or a 200ul tip to lift the line from the slide. If you have a Xylene wash step in your protocol – the Xylene wash will remove and wash the GnomePen line away.
  • Store the GnomePen or BarrierPaste in a cool dark place.

Don’ts

  • Do not use an alcohol/acetone fixative – Formaldehyde and PFA are recommended.
  • Do not store the pen in direct sunlight.
  • Do not store your pen in a hot area (or near a heat emitting device).
  • Do not use the GnomePen near open fire!

If you have any questions – please ask away: info@invignome.com

Enjoy your pen and good luck with your experiments!

Invignome Team

Skip to content